PRODID:-//hacksw/handcal//NONSGML v1.0//EN BEGIN:VEVENT UID:66491635707441 DTSTAMP:20211020T160000Z LOCATION://地址:\n//\n\ nspeaker:\nMichael Liss, PhD\nIeva Drulyte, PhD\nJens Frauenfeld, PhD\n摘要:\nDate: 2021年10月20日\n\nTime:低温电子显微镜(cryo-EM)正迅速发展成为膜蛋白结构测定的主要工具。然而,为结构生物学提供高质量的膜蛋白是具有挑战性的,往往是一个瓶颈。在本次网络研讨会中,我们展示了赛默飞世尔科技和萨利普罗生物技术的专业知识可以用于膜蛋白药物靶点的基因-结构生成。从基因开始——GeneArt平台包括一个基因到蛋白质服务,只需要一个蛋白质序列,涵盖从基因合成到蛋白质纯化的每一步,包括赛默飞雪专有的哺乳动物和昆虫表达系统Expi293、ExpiCHO或ExpiSF9。这些细胞系形成了膜蛋白过表达的良好基础。由于GeneArt专注于可溶胞内和分泌蛋白的纯化,我们与Salipro Biotech合作,在膜蛋白方面取得了专业知识。Salipro Biotech开发了一种专利的纳米膜平台技术(Salipro®),可在脂质环境中稳定所有类型的完整膜蛋白,使其能够用于治疗性抗体、小分子药物和基于结构的药物设计的药物发现项目。通过DirectMX®方法,Salipro Biotech开发了一种一步法,允许直接从粗细胞中纯化和重组膜蛋白。 Combining Thermo Fisher Scientific and the Salipro® platform technologies can therefore overcome one of the biggest barriers of the cryo-EM workflow. Since the presented workflow yields and validates high-quality proteins, it benefits a wide range of downstream workflows, ranging from structure-based drug design to the development of therapeutic antibodies.\n\n \n\nLearning Objectives\n\n * Discuss the value of GeneArt mammalian and insect expressions for cryo-EM.\n * Describe Salipro Biotech Direct Mx technology for purification and stabilization of membrane proteins.\n * Discuss Cryo-EM for structure determination of membrane proteins.\n\n \n\n \n\nWebinars will be available for unlimited on-demand viewing after live event.\n\n \n\nLabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.\n\n URL;VALUE=URI:// SUMMARY:Membrane Proteins: From gene to cryo-EM structure with Thermo Fisher GeneArt and Salipro Biotech DTSTART:20211020T160000Z DTEND:20211020T170000Z END:VEVENT END:VCALENDAR