PRODID:-//hacksw/handcal//NONSGML v1.0//EN BEGIN:VEVENT UID:67051633901093 DTSTAMP:20211013T170000Z LOCATION://地址:\n//\n\nSpeaker:\ nane Jaafar, Ph.D\n\ n摘要:\nDate: 2021年10月13日\n\nTime:临床相关应用,如体细胞变异检测,继续推动现有的下一代测序(NGS)技术的边界,需要高度可扩展的解决方案,与广泛的DNA输入兼容,并提供越来越高的灵敏度和特异性。这些工作流程中的一个持久瓶颈是基因组DNA片段。超声波是目前无偏性DNA片段的黄金标准,但它需要前期设备投资,不容易自动化或规模化,而且由于固有的样本损失,很难应用于低输入样本。目前的酶DNA片段产品缓解了许多这些问题;然而,由于碎片化过程中形成的序列伪影的普遍存在,它们带来了数据质量的挑战,这种伪影会使变体调用复杂化并降低准确性。通过复杂的酶工程和多维实验设计的方法来优化系统,手表制造商基因组学已经开发出手表制造商DNA片段准备库。该工作流利用了酶裂解的过程好处,同时减轻了相关人工制品的形成,包括嵌合读和假snv集中在片段末端的部分回文。此外,包括Equinox聚合酶提供超高保真度,低偏倚文库扩增。 Results highlight robust and tunable fragmentation performance, even with sub-nanogram input amounts and formalin-fixed paraffin-embedded (FFPE) samples, along with high library conversion efficiency. Watchmaker’s library prep solution enables clinical and translational applications to access meaningful insights from a broad range of biological sample types with an unparalleled combination of accuracy and scalability.\n\n\n \n\nLearning Objectives\n\n * Identify sequencing artifacts, such as interchromosomal mate pairs and hairpin artifacts, that can be introduced during library construction and how they can negatively impact assay sensitivity in clinically relevant applications, such as germline and somatic mutation calling.\n * Illustrate the utility of combining a novel enzyme engineering platform and a multidimensional Design of Experiments (DOE) optimization approach to developing workflows that mitigate sequence artifacts.\n * Demonstrate the value of a streamlined, scalable, and highly accurate library preparation workflow for high-stringency applications and challenging samples types, such as ultra-low and degraded inputs.\n\n \n\n \n\nWebinars will be available for unlimited on-demand viewing after live event. \n\n \n\nLabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety. URL;VALUE=URI:// SUMMARY:Overcoming sequence artifacts to fuel clinically relevant NGS applications DTSTART:20211013T170000Z DTEND:20211013T180000Z END:VEVENT END:VCALENDAR