BEGIN:VCALENDAR VERSION:2.0 PRODID:-//hacksw/handcal//NONSGML v1.0//EN BEGIN:VEVENT UID:67101637273652 DTSTAMP:20211026T180000Z LOCATION://www.androidjugaad.com/webinar/constructing-spatially-resolved-single-cell-atlas-mouse-retina-merscope-platform描述:位置:\n//www.androidjugaad.com/webinar/constructing-spatially-resolved-single-cell-atlas-mouse-retina-merscope-platform\n\nSpeaker:\nRui Chen, b.s., Ph.D \n\ nDate: 2021年10月26日\n\nTime:视网膜是视觉系统的感光部分,由6种神经元类型和几种非神经元细胞类型组成,根据细胞形态、功能和分子标记估计有超过100种亚型。最近的单细胞转录组研究表明,小鼠视网膜的大多数细胞亚型都可以根据它们的转录组进行识别。此外,由转录组定义的细胞亚型与从形态和功能获得的结果密切相关。然而,目前单细胞转录组技术的一个主要缺点是需要隔离单细胞,导致空间信息丢失。遗憾的是,空间信息对于许多生物学过程的研究都是至关重要的,如表征神经元回路、发育和退化。因此,为了深入了解视网膜功能的机制,构建一个空间分辨的视网膜单细胞图谱是必要的。我们使用Vizgen公司的MERSCOPETM平台,利用MERFISH对小鼠视网膜进行单细胞空间转录组分析。视网膜的细胞组织具有独特的特征,许多细胞亚型组织在一个紧凑的区域内。 As a result, to generate the spatial map of the retina, it is necessary to optimize the procedure. For example: 1) Due to the compactness of the retina, a new protocol needs to be developed that allows antibody staining against cell membrane along with MERFISH probes against transcripts to improve cell segmentation; 2) Cell segmentation algorithm needs to be trained and optimized for the retina; 3) As some of the cell subtypes are extremely rare ( URL;VALUE=URI://www.androidjugaad.com/webinar/constructing-spatially-resolved-single-cell-atlas-mouse-retina-merscope-platform SUMMARY:Constructing a Spatially Resolved Single-cell Atlas of the Mouse Retina with the MERSCOPE Platform DTSTART:20211026T180000Z DTEND:20211026T190000Z END:VEVENT END:VCALENDAR
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