PRODID:-//hacksw/handcal//NONSGML v1.0//EN BEGIN:VEVENT UID:67121633970400 DTSTAMP:20211014T140000Z LOCATION://www.androidjugaad.com/webinar/heterologous-protein-engineering-characterization-native-regulatory-elements-i-e-coli-i-using-massiv地址:\n//www.androidjugaad.com/webinar/heterologous-protein-engineering-characterization-native-regulatory-elements-i-e-coli-i-using-massiv\n\nSpeaker:\nTyson Shepherd, PhD\n\ n摘要:\nDate: 2021年10月14日\n\nTime:外源蛋白在模式生物中的表达有许多应用,包括蛋白质工程、工业酶和治疗学的生产以及结构-功能发现。外源蛋白通常从质粒中表达,以促进克隆和基因操作。然而,质粒表达有许多缺点,如稳定性降低和对基因拷贝数的控制有限。通过CRISPR编辑,染色体基因整合和后续编辑变得更加容易,从而可以全面检查蛋白质的表达和功能。在本次网络研讨会中,我们演示了如何使用OnyxTM平台为染色体表达的绿色荧光蛋白(GFP)生成大肠杆菌饱和诱变文库。我们鉴定了几个荧光增强或光谱特征改变的工程变种,包括文献验证和新的变种。我们还生成了所有预测的大肠杆菌启动子和核糖体结合位点(RBS)的文库,并使用工程高表达GFP报告系统量化了它们的强度。这些高通量实验深入了解了天然调控序列,并直接在宿主基因组中展示了异种蛋白工程的优势。\n\n \n\nLearning Objectives\n\n \n\n * \n Generate massively parallel genomic libraries for heterologous protein engineering in E.coli\n \n * \n Apply fluorescence-based assays for high-throughput novel variant discovery and investigations into genomic regulatory elements\n \n * \n Identify novel green fluorescent protein (GFP) variants with altered spectral characteristics and improved activity\n \n * \n Evaluate expression of native promoter and ribosomal binding site (RBS) sequences using a stable chromosomal GFP reporter system\n \n\n \n\n \n\nWebinars will be available for unlimited on-demand viewing after live event. \n\n \n\n \n\nLabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety. URL;VALUE=URI://www.androidjugaad.com/webinar/heterologous-protein-engineering-characterization-native-regulatory-elements-i-e-coli-i-using-massiv SUMMARY:Heterologous Protein Engineering and characterization of native regulatory elements in E. coli using Massively Parallel CRISPR Editing DTSTART:20211014T140000Z DTEND:20211014T150000Z END:VEVENT END:VCALENDAR
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